Cupriavidus metallidurans strains, which belong to the Burkholderiaceae family and are characterized by multiple metal resistances, have been mostly isolated from industrial sites linked to mining, metallurgic and chemical industries. These strains are often typified by their large arsenal of mobile genetic elements (plasmids, transposons, genomic islands) that carry determinants allowing the strains to specifically adapt to their environment. For example, C. metallidurans type strain CH34 carries many determinants for metal resistance on its megaplasmids pMOL28 and pMOL30. Interestingly, while they often thrive in oligotrophic niches like industrial sites but also water systems (such as drinking water systems and even spent nuclear fuel pools), Cupriavidus isolates are increasingly being recovered from medically-relevant sources.
Studies at SCK•CEN underscored the rapid evolution of C. metallidurans strains towards significantly increased metal resistance. Surprisingly, the canonical mechanisms did not always participate in this adaptive evolution. In contrast, uncharacterized systems or unidentified transcriptional networks were discovered.