Transcriptomic, proteomic and metabolic changes of Lactobacillus reuteri under simulated microgravity

SCK•CEN Mentor

Mastroleo Felice, fmastrol@sckcen.be, +32 (0)14 33 23 88

Expert group

Microbiology

SCK•CEN Co-mentor

Leys Natalie , nleys@sckcen.be , +32 (0)14 33 27 26

Introduction

Very few publications can be found in the literature that address the issue of spaceflight effects on the intestinal microbiota and in particular on probiotic bacteria. The latter may be administered to space crew for the purpose to help and improve both innate and adaptive immune responses and in the same time prevent or reduce these effects. Probiotics, in fact, are a promising alternative to overcome microbial dysbiosis in space, particularly in situations where the GI flora has been significantly damaged, for example following antibiotic treatment.

Objective

During this study the response of the probiotic Lactobacillus reuteri to simulated microgravity will be tested using both the random positioning machine and the rotating wall vessel facilities. Further samples processing will include transcriptomic (RNA-Seq), proteomic (LC-MS) as well as phenotypic analysis. 

The minimum diploma level of the candidate needs to be

PhD

The candidate needs to have a background in

Bio-engineering , Biology

Estimated duration

1 week